Enhancing Paracetamol with Silver Metal Ions

Enhancing Paracetamol with Silver Metal Ions 1- Introduction The paracetamol (Fig. 1) is one of the famous analgesic drugs which is commonly named as N-acetyl-4-amino-phenol. Paracetamol has an antipyretic medication with little toxicological side effect [1-7]. The fluorometry [8], luminescence [9], electrochemical [10], nuclear magnetic resonance, mass spectroscopy [11] and liquid chromatography [12] are different analyses techniques used to determination of paracetamol drug in pure and pharmaceutical fashions. Paracetamol has a side effect as hepatotoxic in man and animals if it was taken with over dose [13-17]. Herein in this research paper, we aimed to enhance the efficiency of the drug in the presence of silver metal ions by formation of new complex. This complex was proven using spectroscopic and thermo gravimetric analyses. Fig. 1: Chemical structure paracetamol 2- Experimental 2-1- Materials Reagents (paracetamol and silver(I) nitrate) are in analytical grade and used without further purification. 2-2- Instrumentations The elemental analysis (%carbon, %hydrogen and %nitrogen) results were measured using CHN-2400 Perkin Elmer analyzer instrument. FT-IR spectra were scanned on Bruker FT-IR spectrophotometer within 4000-400 cm-1 region. The UV-vis. spectra of reactants and silver(I) complex were scanned using Perkin-Elmer Lambda 4B spectrophotometer in dimethyl sulphoxide solvent. The thermo gravimetric analyses TG/DTG were carried out in nitrogen atmosphere using Schimadzu TGA-50H thermal analyzer. 2-3- Synthesis The 1 mmol of AgNO3 was dissolved in 20 mL distilled water and then mixed to 20 mL of methanolic solution of paracetamol with 1 mmol ratio under magnetic stirring. The pH of mixture was neutralized at 7-8 using diluted ammonium hydroxide solution. The mixture was refluxed at 60 à ¯Ã¢â‚¬Å¡Ã‚ °C and left to evaporate slowly at room temperature. The precipitate was filtered off, washy with hot methanol and dried at 60 à ¯Ã¢â‚¬Å¡Ã‚ °C. 2-3- Microbiological investigation The biological activity of silver(I) complex was tested against bacteria and fungi with more than one test organism. The organisms used in the present investigation including two bacteria (B.subtilis Gram +ve), (E. coli Gram –ve) and two fungi (Aspergillus niger and Aspergillus flarus). The results of microbiological investigations of bacterial and fungi against synthesized silver(I) complex were assessments. 3- Results and Discussion 3-1-Elementl analyses The experimental data of %carbon, %hydrogen and %nitrogen are matched with the calculated values (Table 1). The elemental analyses results are support that NO3à ¯Ã¢â€š ¬Ã‚ ­ ions not detected. The silver(I) complex is air stable with highly melting point > 300 oC. Table 1:Elemental analyses and physical results of Ag(I) complex [Ag2(para)] M.wt (g/mol) %C %H %N %M à ¯Ã‚ Ã…’ (à ¯Ã‚ 1. cm2.mol-1) Found Calcd. Found Calcd. Found Calcd. Found Calcd. 364.88 27.0 26.33 1.87 1.93 4.00 3.84 58.50 59.12 7.0 3-2- Molar conductivity The molar conductance value of the silver(I) complex of paracetamol drug in DMSO solvent with concentration of 1.0010-3 M was found to be 7.00 à ¯Ã‚ Ã…’ (à ¯Ã‚ 1. cm2.mol-1) at 25 à ¯Ã¢â‚¬Å¡Ã‚ °C. This data suggested that silver(I) complex is non-electrolytes. 3-3- Infrared spectra The infrared assignments data of paracetamol free drug and its silver(I) complex are tabulated in Table 2 and silver(I) complex is shown in Fig. 2. Based on the comparison between the infrared spectra of free paracetamol drug ligand and its silver(I) complex, it has been discussed that, the absorption band at 3300 cm-1 and 3200 cm-1 of free paracetamol have been assigned to –OH and –NH stretching vibration motions. These bands have been absence in the spectra of the silver(I) complex due to the involvement in complexation. The strong-to-medium absorption bands at 1650 and 1260 cm-1 in the spectrum of para drug are assigned to the stretching vibration bands of à ¯Ã‚ Ã‚ ®(C=O) and à ¯Ã‚ Ã‚ ®(C-O), respectively. These bands are shifted to lower wavenumbers at 1627 and 1100 cm-1 due to sharing in coordination mode. The presence of new absorption band at 510 cm-1 in silver(I) complex is assigned to à ¯Ã‚ Ã‚ ®(Ag -O) stretching vibration. Upon the infrared spectral assi gnments, the silver(I) ions coordinated to para drug through two place of coordination (deprotonated of –OH) and (deprotonated of –NH and oxygen of carbonyl group) as shown in Fig. 3. Fig. 2: FT-IR spectrum of Ag(I) complex. Table 2: Infrared frequencies within 4000-400 cm-1 of para and its Ag(I) complex. Compound à ¯Ã‚ Ã‚ ®(OH) + à ¯Ã‚ Ã‚ ®(NH) à ¯Ã‚ Ã‚ ®(C=O) à ¯Ã‚ Ã‚ ¤(CNH) amide group à ¯Ã‚ Ã‚ ®(C-O) phenyl group à ¯Ã‚ Ã‚ ®(M-O) para 3300, 3200 1650 1560 1260 [Ag2(para)] 1627 1550 1100 510 Fig. 3: Suggested structure of Ag(I) complex. 3-4- UV-vis. spectra The formation of the Ag(I) complex was also confirmed by UV-vis. spectra in DMSO solvent within the 200-600 nm range. It can see that free para has two absorption bands at 300 and 390 nm due to à ¯Ã‚ Ã‚ °-à ¯Ã‚ Ã‚ °* intra-ligand transition of the aromatic ring and n-à ¯Ã‚ Ã‚ °* electronic transition, respectively. 3-5- Thermo gravimetric analyses The heating rate was controlled at 10à ¯Ã¢â‚¬Å¡Ã‚ °C/min under nitrogen environment. The weight loss was scanned from room temperature till 1000 à ¯Ã¢â‚¬Å¡Ã‚ °C. The thermo gravimetric curve of silver(I) complex is shown in Fig. 4. The thermal decomposition of [Ag2(para)] complex exhibit at two step. 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